Four Weeks of Probiotic Supplementation Alters the Metabolic Perturbations Induced by Marathon Running: Insight from Metabolomics

Few data are available that describe how probiotics influence systemic metabolism during endurance exercise. Metabolomic profiling of athletes will elucidate mechanisms by which may confer benefits to the athlete. In this study, twenty-four runners (20 male, 4 female) were block randomised into two groups using a double-blind matched-pairs design according their most recent Marathon performance. Runners assigned 28-days supplementation with multi-strain probiotic (PRO) or placebo (PLB). Following supplementation, performed competitive track race. Venous blood samples and muscle biopsies (vastus lateralis) collected on morning race immediately post-race. Samples subsequently analysed untargeted 1H-NMR metabolomics. Principal component analysis (PCA) identified greater difference in post-Marathon serum metabolome PLB group vs. PRO. Univariate tests 17 non-overlapped metabolites PLB, whereas only seven By building PLS-DA model components, we revealed combinations able discriminate between PRO post-Marathon. PCA demonstrated no discernible treatment groups. conclusion, alters metabolic perturbations induced Marathon. Such findings be related maintaining integrity gut